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El tratamiento con resiniferatoxina exhibe propiedades antiinflamatorias en un modelo murino de inflamación inducida por lipopolisacárido
Resumen
Introducción. La inflamación es una respuesta fisiológica protectora del sistema inmunológico frente a diversos estímulos, tales como la infección o daño celular, la cual, al no resolverse de manera adecuada, puede ser perjudicial para le hospedero. En la búsqueda de nuevas alternativas terapéuticas que inhiban la respuesta inflamatoria, diversos estudios han reportado el uso de diversas moléculas, tales como la resiniferatoxina (RTX), un potente agonista del receptor TRPV1. Objetivo. Evaluar si el tratamiento con RTX exhibe propiedades antiinflamatorias, utilizando un modelo murino de inflamación inducida por LPS. Material y métodos. Se estimularon ratones BALB/c con LPS y posteriormente fueron tratados con dexametasona (DEX), capsaicina (CAP), Bay 11-7082, capsazepina (CPZ) y RTX. Además, otros grupos de ratones fueron estimulados con LPS y luego fueron tratados con Bay 11-7082 y CPZ más RTX. Después del tratamiento, se determinaron cuantitativamente los niveles plasmáticos de PGE2, NO, IL-1β y TNF-α utilizando kits de ELISA. Resultados. El tratamiento con RTX disminuyó de manera significativa (p*<0.05) los niveles plasmáticos de PGE2, NO, IL-1β y TNF-α. Así mismo, se observó que los tratamientos con Bay 11-7082 y CPZ más RTX mostraron un efecto antiinflamatorio sinérgico, observándose una disminución significativa más pronunciada (*p<0.05) en los niveles plasmáticos de TNF-α y PGE2. Conclusión. Estos hallazgos sugieren que el tratamiento con RTX muestra propiedades antiinflamatorias, aparentemente asociadas con la vía de señalización NF-κB, independiente de los receptores TRPV1, colocando a la RTX como un fármaco potencial en el tratamiento de enfermedades inflamatorias.
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