Presence of red complex bacteria and Aggregatibacter actinomycetemcomitans in necrotic primary teeth with periapical abscess in children of Sinaloa, México.

Erika de Lourdes Silva-Benítez, Jesús Eduardo Soto-Sainz, Pamela Alarcón-Romero, Geraldine Gastélum-Rosales, Gloria Yolanda Castro-Salazar, Rosalío Ramos-Payán, José Geovanni Romero-Quintana, Elsa Maribel Aguilar-Medina, Alfredo del Rosario Ayala-Ham, Uriel Soto-Barreras



Introduction: Pulp necrosis is a common reason of root canal treatment in primary teeth. The red complex and Aggregatibacter actinomycetemcomitans (A.a.) have been associated with pulpal pathologies. Objective: the present study was evaluate the prevalence of the red complex and A.a. in dental plaque, root canal and periapical abscesses of primary teeth with necrotic pulp in children from 3-to-9 years old. Materials and methods: Samples of the three sites were preserved and microorganisms were identified by PCR. Results: Twenty-six children (5.5 ± 1.7 years old) were evaluated, 92.3% were positive for bacteria at least in one place. The most prevalent microorganism was T. forsythia (76.9%) and the least T. denticola (11.5%). However, A.a was not found in any sample. Bacterial plaque was the most prevalent place of bacteria (80.7%, p<0.001). Interestingly, T. forsythia was related with bacterial plaque site, while T. denticola with fistula and P. gingivalis with root canal. Conclusion: The bacteria of the red complex (P. gingivalis, T. denticola and T. forsythia) were found in high frequencies in the three evaluated sites. A.a. was not found in any sample. T. forsythia was more prevalent in bacterial plaque site, while T. denticola in abscess and P. gingivalis in root canal.



Introducción: La necrosis pulpar es uno de los principales motivos por los cuales se realizan tratamientos en dentición infantil. El complejo rojo y Aggregatibacter actinomycetemcomitans (A.a.) han sido asociados con patología pulpares. Objetivo: Evaluar la presencia del complejo rojo y A. a. en placa bacteriana, canal radicular y abscesos periapical en dentición infantil con diagnóstico de necrosis pulpar en infantes de 3 a 9 años de edad. Materiales y métodos: Las muestras de los tres sitios fueron preservadas y los microorganismos fueron identificados por PCR. Resultados: Veinte seis niños (5.5 ± 1.7 años) fueron evaluados, 92.3% fueron positivos para una bacteria en por lo menos uno de los lugares evaluados. El microrganismo más prevalente fue T. forsythia (76.9%) y el menor T. denticola (11.5%). Sin embargo, A.a no fue encontrada en ninguna de las muestras. La placa bacteriana fue el lugar con mayor frecuencia de bacterias (80.7%, p<0.001). Un diagrama simétrico fue realizado dando como resultado que T. forsythia se relacionó con el sitio de placa bacteriana, mientras T. denticola con fistula y P. gingivalis con canal radicular. Conclusiones: Las bacterias del complejo rojo (P. gingivalis, T. denticola y T. forsythia) fueron encontradas en una alta frecuencia en los tres sitios analizados. A.a. no fue encontrada en ninguna muestra. T. forsythia fue la bacteria más prevalente en placa bacteriana, mientras que T. denticola en absceso periapical y P. gingivalis en canal radicular.


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